Research Paper Volume 14, Issue 20 pp 8486—8497

The regulatory mechanism of HSP70 in endoplasmic reticulum stress in pepsin-treated laryngeal epithelium cells and laryngeal cancer cells

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Figure 4. The effect of inhibition of HSP70 on cell viability, apoptosis and autophagy. (A and B) RT-qPCR detected the expression of HSP70 in laryngeal epithelial cells and LC cells. ***p < 0.001 vs. shRNA-NC. (C and D) CCK8 detected the viability in laryngeal epithelial cells and LC cells. (E and F) Apoptosis of laryngeal epithelial cells and LC cells were detected by flow cytometry. (G and H) The autophagy detection kit measured autophagy levels in laryngeal epithelial cells and LC cells. **p < 0.01, ***p < 0.001 vs shRNA-NC+pepsin.