Research Paper Volume 14, Issue 20 pp 8292—8301

Figure 5. Co-overexpression of αS-GCN5 inhibits apoptotic neuronal cell death. (A) Endogenous protein expression of αS in GCN5 overexpressed (oeGCN5) or NC cells. (B) Protein was isolated from NC, αS^+/+ and αS+GCN5 cells and probed for specific proteins. (C) Protein expression of apoptotic markers using lysates from NC, αS^+/+, oeGCN5 and αS+GCN5 cells. (D) Nucleic staining by DAPI where the whitish inclusions (cropped) indicated apoptotic cells with nuclear pyknosis; the pyknosis rate (% chromatin aggregation) was calculated by the scoring percent of cells with nuclear pyknosis in total DAPI-stained cells (scale bar = 40 μm). ^###p < 0.001 compared between vector (vec) vs. αS^+/+ and ^**p < 0.01 compared between αS^+/+ vs. αS+GCN5.