Research Paper Volume 14, Issue 20 pp 8292—8301
α-Synuclein upregulates bim-mediated apoptosis by negatively regulating endogenous GCN5
Figure 4. Inhibition of GCN5 by αS triggering Bim expression. (A) Co-immunoprecipitation of GCN5 and E2F1 in neuronal SH-SY5Y cells and probed for anti-αS. (B) GCN5 siRNA was used to knockdown GCN5 in SH-SY5Y cells; protein lysates were collected 24 h after siGCN5 induction and probed for anti-GCN5 and anti-αS. (C) Protein expression of apoptotic and anti-apoptotic markers.