Figure 2. Overexpression of αS promotes Bim-mediated apoptotic pathway. (A) mRNA and (B) protein expression of Bim and its transcription factors in non-transfected cells (NC) and transfected (αS+/+) cells. (C) SH-SY5Y cells were co-immunoprecipitated by anti-E2F1 and anti-Egr1 or A/G beads only (No 10 ab) as negative control and probed for anti-αS. (D) Protein expression of apoptotic markers.