Research Perspective Volume 14, Issue 20 pp 8150—8166

Figure 2. ZMPSTE24 protein expression during murine aging. ZMPSTE24 protein levels were determined by Western blots from brain, liver, heart, gastrocnemius, and subcutaneous fat of young (9 months of age, n = 5) and old (22 months of age, n = 5) female mice. Relative ZMPSTE24 levels (normalized to GAPDH) were quantified by densitometry using ImageJ software (http://rsb.info.nih.gov/ij/). All bars represent mean ± SEM. The statistical significance of differences between two groups (as indicated with P values) was determined using unpaired, two-tailed Student’s t-test. The antibody used was a Rabbit polyclonal to ZMPSTE24 from Abcam; Catalog number ab38450.