Figure 2.ZMPSTE24 protein expression during murine aging. ZMPSTE24 protein levels were determined by Western blots from brain, liver, heart, gastrocnemius, and subcutaneous fat of young (9 months of age, n = 5) and old (22 months of age, n = 5) female mice. Relative ZMPSTE24 levels (normalized to GAPDH) were quantified by densitometry using ImageJ software (http://rsb.info.nih.gov/ij/). All bars represent mean ± SEM. The statistical significance of differences between two groups (as indicated with P values) was determined using unpaired, two-tailed Student’s t-test. The antibody used was a Rabbit polyclonal to ZMPSTE24 from Abcam; Catalog number ab38450.
