Research Paper Volume 14, Issue 20 pp 8346—8356

Figure 1. Par inhibits the metastatic, invasive and glycolytic abilities of H22 cells. (A) Cell viability assay revealed that Par could inhibit the viability of H22 cells in a dose-dependent manner. ^*P < 0.05 vs. DMSO group. (B–D) Transwell assay found that Par could inhibit the metastasis and invasion of H22 cells, as manifested by significantly reduced numbers of metastatic and invasive cells. ^*P < 0.05 vs. DMSO group. (E) According to the results of clone formation assay, Par inhibited the cell clone formation, leading to reduced number of colonies as compared to the DMSO group. (F) After Brdu staining, many positive cells were present in the DMSO group, indicating that H22 cells were in the proliferative phase. Par group exhibited significantly lower number of positive cells than the DMSO group. Par could inhibit the H22 cell proliferation. (G–I) Par suppressed the glycolytic ability of cells, inhibited the cellular absorption of glucose, lowered the intracellular glucose level and decreased the lactate release in a dose-dependent manner. ^*P < 0.05 vs. DMSO group.