Research Paper Volume 14, Issue 19 pp 8077—8094
Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targets
Figure 3. 2JY-OBZ4 induced tau dephosphorylation in HEK293/tau cells via upregulating the activity of PP2A. (A) Western blots and (B–G) quantitative analysis for Tau-pT181, Tau-pS262, Tau-pS396, Tau, PP2Ac, PP2Ac-pY307 (PP2Ac phosphor-Tyr307, an indicator of inhibition of PP2A), GSK-3β, GSK-3β-pS9 (GSK-3β phosphor-Ser9, an indicator of inhibition of GSK-3β) in HEK293-hTau cells. PP2Ac represents catalytic subunit of PP2A holoenzyme. MW Molecular weight. n = 3 per group. (H) PP2A activity was detected in HEK293-hTau cells. n = 4 per group. (I) GSK-3β activity was detected in HEK293-hTau cells. n = 3 per group. p value significance is calculated from a one-way ANOVA, data are represented as mean ± SEM. *p < 0.05, ** p < 0.01, **** p < 0.0001, compared to controls and # p < 0.05.