Figure 5.PYCR1 mitigates the carcinogenic effect of STAT3. (A) Overexpression of STAT3 [STAT3(OE)] T24 and EJ cells were transfected with PYCR1 siRNAs [STAT3(OE) + si-PYCR1]. Cell proliferation were determined by CCK8 essay in NC, SENP3(OE), SENP3(OE) + si-STAT3 T24 and EJ cells. [mean ± S.D. (error bars), n = 3. **p ≤ 0.01; ***p ≤ 0.001, compared with NC group; #p ≤ 0.05; ##p ≤ 0.01; ###p ≤ 0.001, compared with STAT3(OE) group, two-way analysis of variance]. (B) Cell invasion determined by trans well staining in NC, STAT3(OE), STAT3(OE) + si-PYCR1 T24 and EJ cells. (C) Analysis of cell migration. All data in this figure are represented as mean ± SD. *P < 0.05, compared in T24 cells; #P < 0.05, compared in EJ cells. (D) EDU positive cells in in NC, SENP3(OE), SENP3(OE) + si-STAT3 T24 and EJ cells determined by Confocal immunofluorescence. (E) Abundance of the indicated protein was analyzed by Western blotting. All experiments were performed in triplicates.
