Research Paper Volume 14, Issue 20 pp 8205—8220

Figure 6. Pathway-based gene networks with differences in Sirt1 binding in PFC chromatin between shorter- and longer-lived elder animals. Significant differences in Sirt1 bind peak binding and intensity were introduced in GeneMania for fold-change visualization regardless of genomic region between pooled shorter-lived versus longer-lived elder PFCs. A network of interacting genes consistent in all animals was subjected to JActiveModules for the identification of pathway based modular clusters with minimal overlap. Two pathway -based modular clusters were identified: (A) Module 1 (detail in Supplementary Material Module 1 and Table 3). (B) Module 2 (detail in Supplementary Material Module 2 and Table 4). Tones of yellow indicate increase, and tones of blue indicate decrease in Sirt1 peaks in shorter-lived animals compared to longer-lived ones, indicating differences in silencing between the groups.