Research Paper Volume 14, Issue 19 pp 7972—7985
DAZAP1 facilitates the alternative splicing of KITLG to promote multiple myeloma cell proliferation via ERK signaling pathway
Figure 5. DAZAP1 triggers alternative splicing of KITLG mRNA in MM cells. (A) AS events were classified into five categories: skipped exon (SE), alternative 5′ splice site (A5SS), alternative 3′ splice site (A3SS), mutually exclusive exon (MXE), and retained intron (RI). (B) Binding of DAZAP1 was close to the 3′ splice site. (C) The typical motif on the peak-bound mRNA regions. (D) Schematic diagrams showed the alternative splicing of KITLG. (E and F) Two different probes of KITLG corresponded to different patient survivals in TT3 cohort. (G and H) RNA levels of different isoform of KITLG were tested by PCR and qPCR assays.