Research Paper Volume 14, Issue 19 pp 7906—7925
Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis
Figure 1. Pseudogene UBE2MP1 was anomalously transcripted in HCC cell lines and tissues. (A) Analysis of the dreamBase datasets. The heatmap indicated the expression profile of the transcript of UBE2MP1 in pan-cancer. (B) Analysis of the data from the LCLE. Pseudogene UBE2MP1 is transcriptionally activated and presents a high expression in HCC. (C) RT-qPCR assay demonstrated a significantly high expression of UBE2MP1 transcript in three HCC cell lines, in comparison with the control LO2 cells barely transcribed this pseudogene (**P<0.01). (D) RT-qPCR assay was conducted on 93 real patients’ specimens. The UBE2MP1 transcript was significantly highly expressed in tumor tissues, and only very few detectable UBE2MP1 transcript was found in the non-cancerous tissues (**P<0.01). (E) Statistic of the number of cases concerning the expression of UBE2MP1 transcript in HCC specimens. UBE2MP1 transcript is detectable and highly expressed in most of the tumor tissues (79/93), and was not detected in most of the adjacent non-cancerous tissues (88/93) (P<0.01).