Research Paper Volume 14, Issue 20 pp 8179—8204
Crosstalk between age accumulated DNA-damage and the SIRT1-AKT-GSK3ß axis in urine derived renal progenitor cells
Figure 2. UdRPCs show decline of mesenchymal stem cell characteristics and an increase of DNA-damage with increase donor age. UdRPCs from donors aged between 21 and 77 years were isolated and stemness status was confirmed by immunofluorescent staining for the renal progenitor marker SIX2 (A) (scale bars: 100 μm). mRNA expression of SIX2 and the stem cell proliferation marker CD133 was determined by quantitative real time PCR (B). RT-PCR analysis reveal Progerin transcripts in UdRPCs (C). UdRPCs from donors aged between 21 and 77 years were isolated and DNA damage was visualized by immunofluorescence-based staining for phosphorylated Histone2A.X (pH2A.X) (D). mRNA expression of ATM was determined by quantitative real time PCR (E).