Research Paper Volume 14, Issue 18 pp 7505—7516

Figure 5. ZCCHC12 contributed to OS progression through PI3K/AKT signaling pathway. (A) The expression levels of PI3K/AKT signaling pathway-associated proteins were detected by western blot analysis. (B–I) functional rescue experiments using PI3K agonist 740Y-P or PI3K inhibitor LY294002 were performed to verify the effect of ZCCHC12 on proliferation and migration in OS. ZCCHC12-silenced HOS cells were treated with 740 Y-P while ZCCHC12-overexpressed 143B cells were treated with LY294002, then cells were used for proliferation analysis (B and C), CCK-assays; (D and E), colony formation assays) and migration analysis (F–I), Transwell migration assays and wound-healing assays). scale bar: 200 μm. n = 5; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.