Research Paper Volume 14, Issue 17 pp 7126—7136

Figure 4. Zbtb34 binds to the telomere DNA. (A) The ZnFs within Zbtb34 of mESCs are analyzed through telomere co-immunoprecipitation. Schemata represents the construct of the Zbtb34-EGFP vector and the alanine substitution of ZnF1, ZnF2 or ZnF3. The results show that Zbtb34 is co-immunoprecipitated with the sequence (TTAGGG) of the telomeres. The association of ZnF1, ZnF2 or ZnF3 with telomere DNA is further analyzed. The results reveal that ZnF3 is co-immunoprecipitated with the telomere DNA. The random sequence (TGTGAG) is the control. (B) Fluorescence in situ hybridization shows the localization of Zbtb34 (green) and telomeres (red). The dots in the white boxes indicate the merger of green and red dots. Abbreviations: ZnF: zinc finger; mESCs: mouse embryonic stem cells.