Realgar (As4S4), a traditional Chinese medicine, induces acute promyelocytic leukemia cell death via the Bcl-2/Bax/Cyt-C/AIF signaling pathway in vitro

Zonghong Li; Ruiming Zhang; Xuewei Yin; Nana Li; Siyuan Cui; Teng Wang; Xing Tan; Mingyue Shen; Yun Guo; Jinxin Wang; Dadong Guo; Ruirong Xu

doi:doi:10.18632/aging.204281

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Research Paper Volume 14, Issue 17 pp 7109—7125

Realgar (As₄S₄), a traditional Chinese medicine, induces acute promyelocytic leukemia cell death via the Bcl-2/Bax/Cyt-C/AIF signaling pathway in vitro

Figure 8. Evaluation of the Bcl-2, Bax, Cyt-C and AIF protein levels. Western blot analysis was performed in order to determine the Bcl-2, Bax, Cyt-C and AIF protein levels in NB4 cells after treatment with different concentrations of realgar (0, 12.5, 25.0, 50.0 and 75.0 μg/ml, respectively) for 24, 48 and 72 h, and three independent experiments were repeated. (A) Bcl-2, Bax, Cyt-C and AIF protein levels; (B) Histogram analysis of the target protein expression levels in NB4 cells. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 vs. relevant control samples.