Research Paper Volume 14, Issue 17 pp 7109—7125

Realgar (As4S4), a traditional Chinese medicine, induces acute promyelocytic leukemia cell death via the Bcl-2/Bax/Cyt-C/AIF signaling pathway in vitro

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Figure 7. Determination of the target protein levels. NB4 cells were treated with or without realgar (75.0 μg/ml) for 72 h. The levels of Bcl-2, Bax, Cyt-C, and AIF were measured using immunofluorescence. (A) Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; blue). (B) The fluorescence intensity of each protein is shown. Original magnification, × **P < 0.01.