Research Paper Volume 14, Issue 17 pp 7093—7108
Tyrosine kinase receptor RON activates MAPK/RSK/CREB signal pathway to enhance CXCR4 expression and promote cell migration and invasion in bladder cancer
Figure 3. Overexpression of CXCR4 restores the inhibition of cell migration and invasion caused by RON reduction. 5637 cells GV141/CXC4 or control were added to shRON (+) or shNC (-). The wound healing assay was performed to evaluate the capability of cell migration (A, B). The transwell assay was used to detect the cell invasion ability (C, D). Cell extracts were subjected to Western blot analysis using the indicated antibodies to CXCR4, RON, E-cadherin, and vimentin, respectively (E). Data are shown as mean ± SEM, from one of 3 independent experiments. *P<0.05, versus absence of GV141/CXCR4 and shRON treatment, #P < 0.05, versus GV141/CXCR4 treatment.