Research Paper Volume 14, Issue 17 pp 7093—7108

Figure 2. Ectopic expression of RON reverses the reduction of CXCR4 and the inhibition of cell migration and invasion by siCXCR4. After BIU87cells were transfected with siCXCR4 in the absence(-) or presence(+) of GV230/RON, wound healing assay for analysis of the cell migration (A, B), transwell invasion assay analysis of the cell invasion (C, D) and Western blot analysis of CXCR4, RON, E-cadherin and Vimentin expression (E). After down-regulation of CXCR4 with siCXCR4, the CXCR4 expression was significantly reduced, cell migration and invasion were also inhibited. However, overexpression of CXCR4 in BIU87 cells co-transfected with GV230/RON reversed CXCR4-reduced protein levels, cell migration and invasion. Data are shown as mean ± SEM from one of three experiments with similar results. *P < 0.05, versus absence of GV230/RON and siCXCR4 treatment, ^#P < 0.05, versus siCXCR4 treatment.