Research Paper Volume 14, Issue 19 pp 7650—7661
Senolytic elimination of senescent macrophages restores muscle stem cell function in severely dystrophic muscle
Figure 5. In vivo results of fisetin administration in mdx/utro(−/−) mice. (A) mdx/utro(−/−) mice were treated with fisetin via oral gavage (20 mg/kg for 5 consecutive days each week) for 4 weeks and muscle tissues were analyzed. Immunofluorescent staining of CD68 showed that the number of macrophages was decreased with fisetin administration; staining of SA-β-Gal showed that the number of senescent cells was decreased with fisetin administration; immunofluorescent staining of Pax7 showed that the number of MPCs was increased with fisetin administration; trichrome staining of collage deposition (fibrosis formation) showed that the amount of fibrosis formation was decreased with fisetin administration. (B) Statistics of in vivo results. n = 8 mice/group. Data are presented as mean +/− SD.