Research Paper Volume 14, Issue 17 pp 7003—7013
UBE2C mediated radiotherapy resistance of head and neck squamous cell carcinoma by regulating oxidative-stress-relative apoptosis
Figure 3. UBE2C confers radiotherapy resistance of HNSCC by regulating oxidative-stress-induced apoptosis through 4-HNE signaling. (A) Western blot results of proliferation relative protein (CDK1), anti-apoptosis relative protein (Bcl-2) and apoptosis relative protein (C-PARP, BAX) in Si-UBE2C/Cont CAL27 cells under 0/2 Gy radiation. (B) RT-PCR results of CRP gene expression in Si-UBE2C/Cont CAL27 cells under 0/2 Gy radiation after 24h. (C) RT-PCR results of NOX4 gene expression in Si-UBE2C/Cont CAL27 cells under 0/2 Gy radiation after 24h. (D) Western blot results of oxidative stress relative protein (4-HNE and NADPH) in Si-UBE2C/Cont CAL27 cells under 0/2 Gy radiation. (E) ROS level in Si-UBE2C/Cont CAL27 cells under 0/2 Gy radiation. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control.