Research Paper Volume 15, Issue 24 pp 15624—15639
A regulatory circuit of lncRNA NLGN1-AS1 and Wnt signalling controls clear cell renal cell carcinoma phenotypes through FZD4-modulated pathways
Figure 5. NLGN1-AS1 is associated with the activity of the Wnt/β-catenin pathway and suppressed the expression of FZD4. (A) Kyoto Encyclopedia of Genes and Genomes analysis revealed that the Wnt signaling pathway was significantly altered in ccRCC. (B) The effect of NLGN1-AS1 on the Wnt/β-catenin signaling pathway was analyzed by western blotting with the indicated antibodies and samples from the 769-P and Caki-1 cells transfected with sh-NLGN1-AS1 or sh-NC. The data represent the mean ± SD of 3 replicates. *P < 0.05; **P < 0.01.