Research Paper Volume 15, Issue 24 pp 15624—15639
A regulatory circuit of lncRNA NLGN1-AS1 and Wnt signalling controls clear cell renal cell carcinoma phenotypes through FZD4-modulated pathways
Figure 3. Reduced expression of NLGN1-AS1 markedly suppressed the proliferation of ccRCC cells in vivo. (A) The in vivo effect of NLGN1-AS1 was evaluated in xenograft mouse models bearing tumors originating from 769-P cells stably transfected with sh-NLGN1-AS1 or sh-NC, n = 5/group; Tumor volume and weight of the xenograft were shown in the right. (B) Tumor volume of the xenograft in each group. (C) Tumor weight of the xenograft in each group. (D) The tumor sections from sh-NLGN1-AS1 and sh-NC group of xenograft mouse models were subjected to H&E staining and immunohistochemistry staining using antibodies against ki-67 and PDZ4 (400×). The data represent the mean ± SD of 3 replicates. *P < 0.05; **P < 0.01.