Research Paper Volume 15, Issue 24 pp 15624—15639
A regulatory circuit of lncRNA NLGN1-AS1 and Wnt signalling controls clear cell renal cell carcinoma phenotypes through FZD4-modulated pathways
Figure 2. Reduced expression of NLGN1-AS1 markedly suppressed the proliferation of ccRCC cells in vitro. (A) qRT-PCR was conducted to verify the relative expression of NLGN1-AS1 in 769-P and Caki-1 cells transfected with independent shRNA targeting NLGN1-AS1. (B) CCK-8 assay performed with 769-P and Caki-1 cells after transfection of sh-NLGN1-AS1 compared with sh-NC. (C) Representative results of the colony formation of 769-P and Caki-1 cells transfected with sh-NLGN1-AS1 or sh-NC. The colonies were counted and captured. (D) Representative images of EDU assay performed with the 769-P and Caki-1 cells transfected with sh-NLGN1-AS1 or sh-NC. The data represent the mean ± SD of 3 replicates. *P < 0.05; **P < 0.01.