Research Paper Volume 14, Issue 17 pp 6957—6974

Figure 3. Soft substrates inhibited the proliferation of hFFs. (A) GSEA for cell cycle of mRNAs in soft/TCP. Enrichment score of cell cycle genes against their expression profile of soft/TCP. The x axis was the level, and the y axis was the enrichment score of these genes (left). Heat map showed the distribution of mRNAs expression of soft/TCP enriched under the cell cycle KEGG entry in all samples under the gene set, with each row representing one sample. Each line represented a gene, the color from blue to red represented mRNA expression from low to high (right). (B) Immunostaining against Ki67 of soft and TCP with or without TGF-β1 on day 1. Scale bars: 50 μm. (C) The positive rate of Ki67 in B. ^***P < 0.001 (mean, n = 3 randomly selected fields from triplicate samples). (D) Detection of cell cycle by flow cytometry for soft and TCP with or without TGF-β1. (E) The percentage of PI. ^**P < 0.01; ^***P < 0.001 (mean, n = 3). (F) The percentage of G1-, S-, and G2-phase in the cell cycle. ^*P < 0.05 vs. TCP; ^&&P < 0.01, ^&&&P < 0.001 vs. TCP+ TGF-β1 (mean, n = 3).