Research Paper Volume 14, Issue 16 pp 6594—6604

Figure 5. Effects of miR-375 on cell proliferation are mediated by JAK2. (A) Expression of JAK2 was significantly decreased by siJAK2. (B) The increased cell viability induced by anti-miR-375 was mitigated by Jak2 knockdown. * P < 0.05 vs. anti-miR-375+siJAK2 group. (C, D) Knockdown of JAK2 attenuated the promoting effects of anti-miR-375 on BrdU incorporation (C) and PCNA expression (D). (E) Cell migration enhanced by anti-miR-375 was inhibited by JAK2 knockdown. Scale bar: 100 μm. (F) Expression of JAK2 was increased by the transfection with a recombinant plasmid. (G, H) miR-375-mitigated cell viability (G) and BrdU incorporation (H) was reversed by the reintroduction of JAK2. * P < 0.05 vs. miR-375+JAK2 group. (I, J) The inhibitory effects of miR-375 on PCNA expression (I) and cell migration (J) were attenuated by the restoration of JAK2 expression. Scale bar: 100 μm. All values are represented as the mean ± standard error of the mean. n = 3 per group. *P < 0.05.