Research Paper Volume 14, Issue 15 pp 6255—6268

Figure 5. cMAP4K2-miR-377-VEGFA signaling axis regulates endothelial angiogenic effects. (A–F) HRVECs were treated as shown for 24 h. MTT assays and CCK-8 assays were performed to detect cell viability (A and B, n = 4). Ki67 staining assays were performed to detect cell proliferation. A representative image along with the quantification result were shown. Scale bar: 20 μm (C and D, n = 4). Transwell assay was performed to detect the migratory ability of HRVECs. A representative image and the quantification result were shown. Scale bar: 20 μm (E and F, n = 4). The tube-like structures were observed at 6 h after seeding HRVECs on the Matrigel matrix. The cumulative tube lengths for each field were analyzed. Scale bar: 100 μm (G and H, n = 4). ^*P < 0.05 vs. Ctrl group. ^#P < 0.05 miR-377 mimic + vector vs. miR-377 mimic + VEGFA. The significant difference was analyzed by one-way ANOVA followed by the Bonferroni post hoc test.