Research Paper Volume 14, Issue 15 pp 6255—6268

Figure 1. cMAP4K2 expression is induced in retinal vessels and endothelial cells after high glucose exposure. (A and B) qRT-PCRs were performed to detect the expression of cMAP4K2 and MAP4K2 mRNA in retinal vessels isolated from non-diabetic retinas (non-DR) and diabetic retinas (DR) at 2 month, 4 months, and 6 months (n = 5 animals per group, ^*P < 0.05 vs. Non-DR group, Mann-Whitney U test). (C and D) qRT-PCRs were performed to detect the expression of cMAP4K2 and MAP4K2 mRNA in HRVECs cultured in the medium containing 5 mM glucose, L-glucose (25 mM), or D-glucose (25 mM) for 24 h (n = 4, ^*P < 0.05 vs. 5 mM glucose group; 1-way ANOVA followed by Bonferroni’s post hoc comparison test).