Research Paper Volume 14, Issue 15 pp 6202—6226

Figure 6. SIAH1 inhibits the NF-κB pathway via RPS3 downregulation. (A) Western blotting for NF-κB p65 in A2780 cells separately transfected with Vector, SIAH1, Clt-shRNA and sh-SIAH1 for 48 h. (B) Western blotting for NF-κB p65 in A2780 cells separately transfected with Vector, RPS3, Clt-shRNA and sh-RPS3 for 48 h. (C) Western blotting for SIAH1, RPS3 and NF-κB p65 in A2780 cells separately transfected with SIAH1and sh-SIAH1 for 48 h, and treated with PDTC (200 μM) for 24 h. A2780 cells were separately transfected with SIAH1 and sh-SIAH1 for 48 h and treated with PDTC for 24 h. The number of Cell Colonies was determined (D), the cell number of Transwell assay was obtained (E), the Wound closure percentage was calculated (0, 24, 48, 72 and 96 h) (F), and the Edu positive rates (G), cell viability (H, I left panels), and IC50 for cDDP (H, I right panels) were measured in A2780 cells. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.