Research Paper Volume 14, Issue 15 pp 6202—6226

SIAH1-mediated RPS3 ubiquitination contributes to chemosensitivity in epithelial ovarian cancer

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Figure 3. SIAH1 down-regulates the protein level of RPS3. A2780 cells were separately transfected with SIAH1, RPS3, sh-SIAH1 or sh-RPS3 for 48 h, the number of Cell Colonies was determined (A), the cell number from the Transwell assay was obtained (B), the Wound closure percentage was calculated (0, 24, 48, 72 and 96 h) (C), and the Edu positive rates (D), cell viability (E, F left panels), and IC50 for cDDP (E, F right panels) were measured. *p < 0.05, **p < 0.01, ***p < 0.001.