Research Paper Volume 14, Issue 15 pp 6202—6226

Figure 1. SIAH1 sensitizes ovarian cancer cells to cDDP. (A) Western blotting for SIAH1 in SKOV3 and A2780 cells. (B) Representative images of immunohistochemical staining for SIAH1 in tumour specimens from ovarian cancer patients with PFS > 6 months vs. PFS < 6 months. Scale bar: 200 μm (left) and 100 μm (right). (C) Staining was assessed and scored on a scale of 0 (<5% staining) to 4 (>75% staining). The quantification of IHC staining (n = 24; PFS > 6, n = 12; PFS < 6, n = 12) was shown. The results from a Cell Colony formation assay (D) and Transwell assay (E) of A2780 cells transfected with Vector, SIAH1, Clt-shRNA and sh-SIAH1 were shown. Scale bar: 400 μm. A wound-healing assay was used to assess the effects of SIAH1 on cellular motility over time as shown (0, 24, 48, 72 and 96 h; Scale bar: 400 μm) (F). The results from a Cell Edu assay (Scale bar: 200 μm) (G), Cell Viability (H, I left panels) and IC50 for cDDP (H, I right panels) in A2780 cells were shown.^*p < 0.05, ^**p < 0.01, ^***p < 0.001.