Research Paper Volume 14, Issue 15 pp 6187—6201

Figure 3. Protective effect of Artemisia argyi water extract (AAW) against DNA damage and telomere shortening in doxorubicin induced hADMSC. (A) DNA damage repair ability of Artemisia argyi water extract was determined by using γ-H2AX assay. Double stranded breaks (DSB) foci (Red; marked with yellow arrow) was observed in Doxorubicin induced senescent hADMSC. Artemisia argyi water extract has repaired the DSB foci. (B) The depleted expression of TERT (Green) was restored by Artemisia argyi water extract. (C) Telomere length analysis was performed using qPCR. Average relative telomere length shown as T/S ratios in doxorubicin induced senescent hADMSC before and after treatment with Artemisia argyi water extract (AAW) and compared with untreated control; T : Target(Telomere); S : Single copy gene (hbg). #P<0.05 denotes a significant decrease and *P<0.05 denotes a significant increase in comparison with untreated control.