Research Paper Volume 14, Issue 15 pp 6066—6080
PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling
Figure 3. Overexpression of PDCD10 promotes the proliferation, migration, invasion and EMT of PA cells. (A, B) Western blotting was performed to detect the impact of PDCD10 overexpression on the expression levels of EMT markers in Att-20 cells and TtT/GF cells. Band intensities were quantified and normalized to GAPDH. (C, D) CCK-8 assay was used to assess cell proliferation potential after PDCD10 overexpression in Att-20 and TtT/GF cells. (E, F) Scratch assay was employed to examine the relative migration rates of Att-20 and TtT/GF cells after PDCD10 overexpression (magnification:100x). (G, H) Transwell invasion assay was used to detect the invasion potential of Att-20 and TtT/GF cells after PDCD10 overexpression (magnification: 200x). * P < 0.05.