Research Paper Volume 14, Issue 15 pp 6066—6080
PDCD10 promotes the aggressive behaviors of pituitary adenomas by up-regulating CXCR2 and activating downstream AKT/ERK signaling
Figure 2. PDCD10 silencing suppresses the proliferation, migration, invasion and EMT of PA cells. (A) Western blotting was performed to detect the impact of PDCD10 silencing on the expression of EMT markers in Att-20 cells and TtT/GF cells. (B) Band intensities were quantified and normalized to GAPDH. (C, D) CCK-8 assay was used to assess cell proliferation capacity after PDCD10 silencing in Att-20 and TtT/GF cells. (E, F) Scratch assay was used to examine the relative migration rates of Att-20 and TtT/GF cells (magnification:100x). (G, H) Transwell invasion assay was used to analyze the invasion potential of Att-20 and TtT/GF cells (magnification: 200x). * P < 0.05.