Research Paper Volume 14, Issue 15 pp 6128—6148

Figure 6. αB-crystallin inhibited ATR activation under the UVA irradiation. Both pEGFP-HLE and pEGFP-HαB-HLE cells (Mao et al., 2004) were grown to 90% confluence, then subjected to mock irradiation, 37.5 KJ/m2 and 75 KJ/m2 UVA irradiation, respectively. The irradiated cells were harvested for extraction of total proteins which were used for analysis of total ATR (T-ATR, 300 kd), phosphorylated ATR at S-428 and β-Actin (loading control) by Western blot analysis (A). Quantitative results of the T-ATR, and p-ATR-S428 levels against loading control in pEGFP-HLE cells and pEGFP-HαB-HLE cells (B) were analyzed by Image J software. Note that UVA induced significant upregulation of ATR activity (as reflected by phosphorylation at S428) in pEGFP-HLE cells. In contrast, in pEGFP-HαB-HLE cells, both total ATR and phosphorylated ATR at S428 were downregulated under UVA irradiation. N=3. NS, not significant. *p<0.05.