Research Paper Volume 14, Issue 15 pp 6128—6148

Figure 4. αB-crystallin inhibited p53 hyperphosphorylation under UVA irradiation. Both pEGFP-HLE and pEGFP-HαB-HLE cells (Mao et al., 2004) were grown to 90% confluence, then subjected to mock irradiation, 37.5 KJ/m2 and 75 KJ/m2 UVA irradiation, respectively. The irradiated cells were harvested for extraction of total proteins which were used for analysis of total p53 (T-p53) (A, B), phosphorylated p53 at S-20 (p-p53-S20) (A) and phosphorylated p53 at S-37 (p-p53-S37) (B) by Western blot analysis. Quantitative results of the T-p53, p-p53-S20 (C) and p-p53-S37 (D) levels were analyzed by Image J software. Note that UVA-induced much stronger p53 activity (phosphorylation at S-20 and S-37) in pEGFP-HLE cells than that in pEGFP-HαB-HLE cells. N=3. NS, not significant; *p<0.05, ** p<0.01, *** p<0.001.