Research Paper Volume 14, Issue 15 pp 6128—6148

Figure 1. Establishment of vector and MAB21L1 overexpression clones with mouse lens epithelial cells, αTN4-1 line. The plasmids of P3X-Flag-CMV-10-Vector and P3X-Flag-CMV-10-MAB21L1 were transfected into αTN4-1 cells, respectively. After transfection, the P3X-Flag-CMV-10-Vector-αTN4-1 (Vector-αTN4-1 in short) cells and P3X-Flag-CMV-10-MAB21L1-αTN4-1 (MAB21L1-αTN4-1 in short) cells were screened with 600 μg/ml G418 for 4 weeks, and individual clones were obtained. Clone 1 of vector-αTN4-1 and MAB21L1-αTN4-1 cells were confirmed by Western blot analysis (A). (B) Quantitative results of the MAB21L1 protein expression levels in A by Image J software. N=3. *** p<0.001.