microRNA-497 prevents pancreatic cancer stem cell gemcitabine resistance, migration, and invasion by directly targeting nuclear factor kappa B 1

Qiangfeng Yu; Zhe Xiu; Yizeng Jian; Jianyin Zhou; Xiaopeng Chen; Xiang Chen; Chunxiang Chen; Hongbao Chen; Sijia Yang; Libo Yin; Wenlong Zeng

doi:doi:10.18632/aging.204193

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Research Paper Volume 14, Issue 14 pp 5908—5924

microRNA-497 prevents pancreatic cancer stem cell gemcitabine resistance, migration, and invasion by directly targeting nuclear factor kappa B 1

Figure 7. MiR-497 mimics inhibited NFκB1-induced resistance to gemcitabine in pancreatic cancer cells. CSCs and non-CSCs derived from populations of AsPC-1 or BxPC-3 cells were transfected with the NFκB1 overexpression vector, empty vector alone, or in combination with miR-497 mimics and then exposed to gemcitabine. After 24 h of treatment, cell viability was examined by the CCK-8 assay. **p < 0.01, compared to the Vect + gemcitabine group; #p < 0.05, compared to the NFκB1+gemcitabine group. Vect, empty vector; CSCs, cancer stem cells.