Research Paper Volume 14, Issue 14 pp 5838—5854

Figure 7. Effect of 14-3-3ζ on NSCLC proliferation and sensitivity to chemotherapy. (A) NSCLC cells with different status of 14-3-3ζ were seeded in 96-well plate to evaluate their proliferative activity at indicated time points by the MTT assay. ^*P < 0.05, ^**P < 0.01. (B) Representative images of cell colony assays after regulating the expression of 14-3-3ζ in A549, H292 and SPC-A-1 cells, respectively. (C and D) Western blot analysis of the phosphorylation status of Akt and Erk in NSCLC cells with different status of 14-3-3ζ. β-actin was used as equal loading control. (E) Cells were treated with increasing concentrations of cis-platin for 24 h. After indicated treatment, cell viability was measured by MTT assay. ^*P < 0.05 vs. A549 EV group and ^**P < 0.01 vs. A549 EV group, respectively. (F) Western blot analysis of apoptosis-related proteins after cis-platin treatment in A549 EV and A549 14-3-3ζ cells. β-actin was used as equal loading control.