Figure 6. Overexpression of 14-3-3ζ accelerates LLC cell lung metastasis in vivo. (A and B) The LLC cells were transfected with siRNA targeting 14-3-3ζ or negative control. After indicated treatment, cells were injected into C57BL/6J mice through the medial tail vein. At the end of experiment, mouse lungs were carefully isolated and metastatic lung nodules were calculated. (C) Representative histological and IHC images of mouse lungs after LLC cells tail vein injection. The metastatic LLC nodules and adjacent normal lungs were evaluated for Ki67, 14-3-3ζ, E-cadherin and vimentin expression. Scale bar = 200 μm.