Research Paper Volume 14, Issue 14 pp 5783—5799

Figure 5. CFHR3 downregulation promotes phosphorylation of STAT3 and (A, B) GSEA plots show the enrichment of genes in response to JAK/STAT3 signaling between the HCC tissues with CFHR3^high and CFHR3^low (A), or between the HCC tissues with miR-590-3p^high and miR-590-3p^low (B). (C–G) MHCC-97L cells transfected with shCFHR3-RNAs or miR-590-3p precursor were treated with S3I-201 (50μM) or DMSO (control) for 8 hours, and the indicated protein levels (C), cell growth (D), migration and invasion (E–G) were investigated. (H, I) The indicated luciferase-labeled MHCC-97L cells (2 × 10⁶ cells/mouse) were injected into NOD-SCID mice (H); the luciferase activity was visualized 6 weeks post-transplantation (I, n = 5) (J, K) The tumor size (J) and number (K) of metastatic tumor nodules in mouse lung were observed and shown in a bar chart, respectively. (L) Representative IHC images of mouse metastatic tumors formed by MHCC-97L cells. (Remarks: * represents P < 0.05; ** represents P < 0.01; *** represents P < 0.001; **** represents P < 0.0001; Data are presented as mean ± SD).