Figure 3. Validation of candiate genes up-regulated by SOF. To avalidate the SOF-upregulated genes identified from NGS, the OR-6 cells were treated with SOF (0.1 to 2.5 μM) for 24 hrs. The cells were harvested to determine gene expression with Q-PCR validation of PHOSPHO2-KLHL23, TSNAX-DISC1, TRIM39, RPP21 in OR-6 cells were performed using GAPDH as a normalized control. The experiments were performed from three independent experiments. (*p < 0.05; **p < 0.01; ***p < 0.001).