Figure 12. Validating the effect of CENPA on tumors in vivo. (A and B) Validation of protein expression levels of cancer stem cell biomarkers by western blot and immunofluorescence. (C) Tumor volume curve of control group and knockdown group. (D) Tumor weights in control and knockdown groups. (E) Image of xenograft tumors in different groups of mice. (F and G) Animal imaging technology to detect differences between control and knockdown groups. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).