Research Paper Volume 14, Issue 13 pp 5537—5553

Figure 11. Identification of a risk prognostic gene regulating tumor stemness in lung adenocarcinoma cells in vitro. (A) Application of QPCR to compare CENPA and CCNB1 mRNA expression in tumor cells and normal epithelial cells. (B and C) QPCR and western blot to validate the effect of sh-RNA knockdown. (D–G) The effect of knocking down CENPA on cell proliferation ability was examined by CCK-8, EDU and clone formation respectively. (H) QPCR detection of tumor stem cell biomarkers. (^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001).