Research Paper Volume 14, Issue 13 pp 5523—5536

Figure 4. ZNF674-AS1 exhibited suppressive effects on CHST7 expression. (A, B) The RNA levels of ZNF674-AS1 and CHST7 in AGS and MKN-45 cells stably transfected specific shRNAs against ZNF674-AS1 (sh-ZNF674-AS1-1 and sh-ZNF674-AS1-2) or empty plasmid (shCtrl) were examined by qRT-PCR (C) Protein level of CHST7 in ZNF674-AS1-overexpressing or negative control AGS/OXA and MKN-45/OXA cells was determined by western blot. (D, E) The RNA levels of ZNF674-AS1 and CHST7 in AGS/OXA and MKN-45/OXA cells stably transfected ZNF674-AS1 overexpression plasmid (pSin-ZNF674-AS1) or empty vector (pSin-vec) were examined by qRT-PCR (F) Protein level of CHST7 in ZNF674-AS1-silenced or negative control AGS and MKN-45 cells was examined by western blot. (G) RNA level of CHST7 in oxaliplatin-resistant tumor tissues and oxaliplatin-sensitive tumor tissues. (H) qRT-PCR showed CHST7 expression in six GC cell lines (oxaliplatin-resistant cells or parental MKN-45, AGS and MKN-28) and normal human gastric epithelial cell lines. Student’s t-test.