Research Paper Volume 14, Issue 13 pp 5523—5536

Figure 2. Forced expression of ZNF674-AS1 inhibited oxaliplatin resistance of gastric cancer cells. AGS/OXA and MKN-45/OXA cells were stably transfected with ZNF674-AS1 overexpression plasmid (pSin-ZNF674-AS1) or empty vector (pSin-vec) (A) The overexpression efficiency of ZNF674-AS1 was validated by qRT-PCR. (B–E) Cell viabilities were examined by CCK-8 assay and the IC₅₀ value to oxaliplatin was calculated. (F) Clone formation of ZNF674-AS1-overexpressing or negative control AGS/OXA and MKN-45/OXA cells with the treatment of 15μM oxaliplatin. (G) Cell apoptosis was determined by caspase-3 activity assay, with the treatment of 15μM oxaliplatin. (H, I) mRNA levels of MDR1, MRP1 and LRP1 were evaluated by qRT-PCR. Two-way ANOVA for (B, D) Student’s t-test for others.