Research Paper Volume 14, Issue 13 pp 5493—5510
E3 ubiquitin ligase RBX1 drives the metastasis of triple negative breast cancer through a FBXO45-TWIST1-dependent degradation mechanism
Figure 5. RBX1 facilitates TNBC metastasis and invasion via raising the expression of TWIST1. (A) The expression of TWIST1 and RBX1 was measured with western blotting. The raise of TWIST1 reduced the expression of TWIST1 in MDA-MB-231-shRBX1 cells. (B, C) Transwell analysis suggested that the increase of TWIST1 markedly rescued the cell invasion and migration in the MDA-MB-231-shRBX1 cells (*P<0.05). (D) H&E staining of sections embedded with paraffin of metastatic nodules in the lung. *P < 0.05. (E) Western blotting was applied for measuring the levels of TWIST1 and RBX1 protein. The TWIST1 expression knockdown greatly suppressed the raise in the expression of TWIST1 in SUM159PT-p-RBX1 cells. (F, G) Transwell analysis displayed that TWIST1 suppression attenuated RBX1-enhanced cell invasion and migration. *P < 0.05. (H) H&E staining of sections embedded with paraffin of metastatic nodules in the lung. * P < 0.05.