Figure 6.LncRNA H19 knockdown inhibited OGD/R-induced pyroptosis by downregulating HMGB1. PC12 cells were transfected with sh-H19 alone or together with pcDNA-HMGB1 and then subjected to OGD/R. (A) Relative levels of HMGB1 in transfected PC12 cells following OGD/R exposure. (B–D) The levels of IL-1β, IL-18, TNF-α were determined in transfected PC12 cells following H/R exposure by ELISA. (E–H) The protein levels of NLRP3, ASC, and caspase-1 were determined in transfected PC12 cells by Western blotting. (I) Cell apoptosis rate was identified by TUNEL assay. The results were presented as the mean ± SD. N = 3; *P<0.05.
