Research Paper Volume 14, Issue 14 pp 5699—5709

Increased cytochrome C threonine 50 phosphorylation in aging heart as a novel defensive signaling against hypoxia/reoxygenation induced apoptosis

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Figure 5. Effects of Cytc T-50 phosphorylation on caspase and Bcl-2 family activity. ELISA determination of caspase-9 (A), caspase-3 (C) and caspase-8 activity (E) in cardiomyocytes transfected with lentivirus. Representative Western blot and quantitative levels showing the expression of cleaved caspase-9 (B), cleaved caspase-3 (D), BCL-2 (F), Bax (G), BCL-2/Bax (H) and BCL-XL (I). n=3 in each group. ** P < .01 vs. NC, ## P < .01 vs. WT, && P < .01 vs. T50E.