Research Paper Volume 14, Issue 14 pp 5699—5709
Increased cytochrome C threonine 50 phosphorylation in aging heart as a novel defensive signaling against hypoxia/reoxygenation induced apoptosis
Figure 3. Gene manipulation successfully altered the expression of Cytc variants. (A) Transfection efficiency, 48 hours after lentiviral transfection of cardiomyocytes. Transfection efficiency is indicated by concomitant contrast and fluorescence microscopy. Lentiviral vectors carried GFP gene. Cardiomyocytes infected by Cytc variants-carrying lentivirus are identifiable by fluorescence microscopy 48 hours after infection. (B) Representative immunoblots of Cytc. There were no statistical differences in WT, T50E and T50A.