Research Paper Volume 14, Issue 12 pp 5250—5270

Figure 5. TRPM7 silencing significantly suppresses metastasis in SAS-derived tumor xenograft model in vivo. (A) The tumor curve over time shows that cisplatin and shTRPM7 in combination suppressed tumor growth. (B) The average body weight over time curve demonstrated no apparent systematic toxicity in the mice receiving combined treatment. (C) The Kaplan–Meier survival curve shows that the mice receiving combined treatment had the highest survival ratio compared to the other groups. (D) Immunostaining analysis of tumor sections showed that the combined treatment suppressed NAFTC3 and ki67 expression the most, compared with other groups. **P < 0.01; ***P < 0.001. (E) Bar graph showing the comparison of Q-score for tissue expression in tumor section resulted significant inhibition of NFATC3, ki67, and EMT markers vimentin, snail, and slug. *p < 0.05, **P < 0.01, and ***P < 0.001.